Flow cytometry is a technique for multiparametric analysis of cell subpopulations. Cells are identified with the use of monoclonal antibodies labeled with fluorescent molecules. Flow cytometry can be used to determine the lineage, degree of maturity, and presence of aberrant phenotypes in leukemia cells, to diagnose lymphoproliferative syndromes and acute leukemia, in which analysis of minimal residual disease is valuable for treatment follow-up, and to diagnose paroxysmal nocturnal hemoglobinuria, among other conditions.
We have an extensive panel of monoclonal antibodies conjugated with different fluorochromes. Our laboratory is equipped with three flow cytometers with 3, 4, and 5 fluorescence detectors, and specific software for acquisition and interpretation of the data.