Molecular biology enables identification of genetic abnormalities in tumor cells from leukemia and lymphoma, which is crucial for the diagnosis. It is also used to analyze minimal residual disease and to monitor hematopoietic chimerism following allogeneic stem cell transplantation.
DNA or RNA for molecular analysis can be obtained from peripheral blood, biological fluids, bone marrow, fixed and paraffin-embedded material, and frozen material, among others.
The techniques used include polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR), with subsequent analysis of the PCR product by agarose gel or capillary electrophoresis.
We also use real-time quantitative PCR to analyze gene expression and to quantify minimum residual disease. For this purpose, we have three quantitative PCR instruments that also serve researchers from Hospital Clínic de Barcelona and August Pi i Sunyer Institute for Biomedical Research (IDIBAPS, Institut d'Investigacions Biomèdiques August Pi i Sunyer).