Clínic Barcelona. Hospital Universitari

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Basic profile of nonimmune hemolysis, whole blood

Fee code: 6470

Physician:

Maribel Díaz Ricart, Ana Belén Moreno Castaño

Laboratory:

> Pathology Department

Method description

Molecular absorption spectrometry

Delivery time

15 days

Note to delivery time

Requisites

The samples must arrive from Monday to Thursday, never on Fridays or on the eve of holidays. Indicate age, sex and reason for the request

Specimen type

Whole blood (1 tube with sodium heparin + 1 tube with EDTA)

Specimen volume

8 mL (heparina) + 2 tubos de 5 mL (EDTA)

Specimen minimum volume

4 mL (heparina) + 2 tubos de 1,5 mL (EDTA)

Collection instructions

Without effects of recent transfusion (minimum 1 month)

Preanalytic treatment

---

Transport conditions

At room temperature within 4 hours of collection, or refrigerated at a maximum of 6 hours

Rejection criteria

Clotted sample, Hemolysis

Results

Reference values

Values Units   Age  
Glucose-6-phosphate dehydrogenase 5,7 - 9,9 Ul/g Hb    
Acidified glycerol test > 1800 seconds    
Immediate osmotic resistance 4 - 4,6 g/dL    
Resistance osmóstica incubated 5 - 5,6 g/dL    
Glucose phosphate isomerase 46 - 66 Ul/g Hb    
6-phosphogluconate dehydrogenase 6,2 - 10,2 Ul/g Hb    
Pyruvate kinase (PK) 8,4 - 15,2 Ul/g Hb    
(HK) Hexokinase 0,62 - 1,26 Ul/g Hb    
PK/HK Index 7,2 - 15,6 Ul/g Hb    
Erythrocyte morphology      
Hemoglobinopathies-globin chains View provision      

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Biomedical Diagnostic Center (CDB)

Villarroel, 170 · 08036 Barcelona
Tel: +34 93 227 54 64 · Fax: +34 93 227 98 75 · · www.hospitalclinic.org